These materials include compounds that reduce the wear and tear of rubber tires, and antimicrobials used in households, hospitals and industry for disinfecting or preventing microbial growth.
Now, two projects lead by researchers from the University of Saskatchewan’s (USask) Toxicology Centre have been awarded $300,000 each by Fisheries and Oceans Canada (DFO) to study the toxicological impacts of these “emerging chemicals” on fishes of commercial, recreational and Aboriginal (CRA) importance to Canada.
A team led by Dr. Markus Brinkmann (PhD), assistant professor in USask’s School of Environment and Sustainability (SENS), is studying the toxicological workings and impacts of a chemical known as 6PPD used in rubber tires. It is shed by tires on roadways, becomes oxidized to form 6PPD-quinone, and is then carried to surface waters by stormwater runoff.
An initial study by researchers in Washington State determined that the chemical was deadly to coho salmon at trace concentrations, and Brinkmann’s team is studying its potentially widespread ecological risk to Canadian ecosystems.
The group is researching the impacts of 6PPD-quinone on rainbow trout, arctic char, westslope cutthroat trout, lake trout and fathead minnows in Saskatchewan and Alberta.
“If we understand better the differences in the sensitivity across those fish species, we can conduct better risk assessments,” says Brinkmann. “Another important issue is to find out the mechanisms by which the chemical kills the fish, so that we can help avoid some of those problems in the future.”
Most chemicals are required to undergo a thorough risk assessment before their release, Brinkmann says, so the approval of 6PPD, which has been used in tires since the early 2000s, points to holes in the safety net.
“Our research is going full-throttle already, and we will have interesting findings coming out almost immediately."
Dr. Natacha Hogan (PhD), associate professor in the Department of Animal and Poultry Science at USask’s College of Agriculture and Biosciences, is principal investigator of a project aimed at identifying the biological impacts of antimicrobials on fish that live in waters into which wastewater is released.
Her team is evaluating the impact of two antimicrobials that have emerged to replace triclosan — a product in wide use for decades that has been curtailed because of potential risks to humans and aquatic life. Virtually no fish toxicity information exists in Canada on these emerging chemicals.
“It’s important not only to generate toxicity data and understand the impact on fish, but whether fish species differ in sensitivity and if that needs to be taken into account in determining what we would consider a safe level in waters where fish might be found,” says Hogan.
The project involves rainbow and lake trout, representative of salmonids that are considered important CRA species in Canada, as well as fathead minnows and white suckers, from the cyprinid group of fish found around the globe.
“The chemicals we are studying in the two projects are examples of the many emerging contaminants of concern whose safety needs to be assessed,” says co-principal investigator Dr. Markus Hecker (PhD), USask professor and Canada Research Chair in Predictive Aquatic Ecotoxicology.
“It’s part of a bigger story on how we can develop tests that help us understand how a chemical causes health effects in organisms, because we could then use the information and predict the safety of countless other chemicals that have similar properties.”
Both projects will use a next-generation tool for chemical hazard assessment (EcoToxChips), recently developed by team members as part of a large Genome Canada initiative, to identify early toxicity indicators in and predict the vulnerability of these key target fish species.
Most of the research will be conducted in the Aquatic Toxicology Research Facility at USask, and the funding will support an enriched training experience for several undergraduate and graduate students, a postdoctoral fellow and shared technician, says Hogan.
The projects feature a diverse group of co-investigators, including Dr. Lynn Weber (PhD) and Dr. David Janz (PhD), professors at the Western College of Veterinary Medicine (WCVM); Dr. Alper J. Alcaraz (PhD), a postdoctoral fellow at the Toxicology Centre; and Dr. Steve Wiseman (PhD), an associate professor at the University of Lethbridge.